Biotechnology: Principles & Process Questions

The given segment of DNA has restriction sites I and II, which create restriction fragments a, b and c. Which of the following band pattern produced by electrophoresis would represent the separation & identity of these fragments ?

Read the following statements and find out the incorrect statement (i) Now-a-days the most commonly used matrix is agarose which is a synthetic polymer. (ii) Larger the fragment size, the farther it moves. (iii) We can not see pure DNA in UV light wi

What is the advantage of a sparged stirred-tank bioreactor over a simple stirred-tank bioreactor

Read the following statements regarding nematode resistant tobacco plant. Find the correct order of sequence? A. Parasite could not survive in a transgenic host B. Introduction of DNA that produced both sense and anti- sense RNA in the host cells. C.

Read the following statements. Statement-I : If a recombinant DNA bearing gene for resistance to Ampicillin is transferred into E.coli cells, the host cells become transformed into ampicillin sensitive cells. Statement-II : If we spread above transfo

Read the following statements:A. Since DNA is hydrophilic molecule, it cannot pass through cell membrane. B. Bacteria are made competent to uptake DNA by treating with monovalent cations like calcium. C. To enable the bacteria to uptake recombinant D

The process by which seperated bands of DNA are cut out from the agarose gel and extracted from gel piece is known as

Given below are two statements : Statement-I : Downstream processing includes separation and purification of the product after completion of the biosynthetic stage. Statement-II : The downstream processing and quality control testing is same for all

Choose the incorrect statement regarding Downstream processing:

The first step in the isolation of genetic material is the breakdown of the:

Which gene in pBR322 codes for the proteins involved in the replication of the plasmid?

Agarose is a polymer extracted from:

Which enzyme is used to join DNA fragments?

A series of processes such as separation and purification of products after the biosynthetic stage is called:

A simple Stirred-tank bioreactor typically has:

Stirred-tank bioreactors have been designed primarily for:

Bioreactors are useful in:

The vessels in which raw materials are biologically converted to specific products using microbial or animal cells are:

The ultimate aim of recombinant DNA technology is to produce a desirable:

If a process of replication is repeated 30 times, the DNA can be amplified approximately:

In PCR, 'Annealing' refers to the:

The thermostable DNA polymerase used in PCR is isolated from the bacterium:

The correct sequence of steps in a PCR cycle is:

PCR stands for:

The final step of DNA isolation involves the addition of ______ to precipitate the purified DNA.

RNA can be removed from a DNA mixture by treatment with:

To isolate pure DNA from a fungal cell, the cell wall is digested using the enzyme:

The 'Gene Gun' method, suitable for plants, uses micro-particles of:

In which method is recombinant DNA directly injected into the nucleus of an animal cell?

The method of 'Heat Shock' in transformation involves placing cells on ice, then briefly at:

To make a bacterial cell 'competent' to take up DNA, it is treated with a specific concentration of a divalent cation like:

Retroviruses are used as vectors in animals because:

Which of the following is used as a vector for cloning genes into higher plants?

If a recombinant DNA is inserted within the coding sequence of an enzyme like beta-galactosidase, it results in:

The ligation of alien DNA in pBR322 is generally carried out at a restriction site present in:

Transformation is a procedure through which:

Selectable markers like antibiotic resistance genes are used to:

Which of the following is NOT a feature required to facilitate cloning into a vector?

The sequence responsible for controlling the copy number of the linked DNA in a vector is:

Plasmids are suitable as vectors because:

The process of cutting out and extracting separated DNA bands from the agarose gel is called:

Separated DNA fragments can be visualized only after staining with:

In gel electrophoresis, DNA fragments separate according to their size through the:

The matrix most commonly used in gel electrophoresis is:

DNA fragments are negatively charged molecules; they can be separated by moving them towards the:

The overhanging single-stranded stretches of DNA produced by restriction enzymes are called:

EcoRI cuts the DNA between bases G and A only when the sequence ______ is present in the DNA.

Each restriction endonuclease recognizes a specific:

Exonucleases remove nucleotides from:

Restriction enzymes belong to a larger class of enzymes called: