Provided below are recognition sequences for restriction enzymes 1 (RE1), 2 (RE2) and 3 (RE3). Arrows indicate the positions where the enzymes digest on the two strands. Which of the following can the

Question

Provided below are recognition sequences for restriction enzymes 1 (RE1), 2 (RE2) and 3 (RE3). Arrows indicate the positions where the enzymes digest on the two strands. Which of the following can the RE1 digested DNA ligate to?

Accepted Answer

Answer: All three RE1, RE2 and RE3 digested DNA. — For two pieces of DNA to ligate (stick together), their overhanging single-stranded ends—often called sticky ends—must be perfectly complementary (base-pair matching). Let's look at the sticky ends ea

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