Biotechnology : Principles and Processes
60 questions · Click an option and press Check Answer to reveal the result
Read the following statements.
Statement-I : If a recombinant DNA bearing gene for resistance to Ampicillin is transferred into E.coli cells, the host cells become transformed into ampicillin sensitive cells.
Statement-II : If we spread above transformed cells on agar plates, containing ampicillin, only non-transformed cells will grow, transformed recipient cells will die.
In the light of above statements, find the correct option.
Choose the incorrect statement regarding Downstream processing:
Read the following statements regarding nematode resistant tobacco plant. Find the correct order of sequence?
A. Parasite could not survive in a transgenic host
B. Introduction of DNA that produced both sense and anti- sense RNA in the host cells.
C. Using Agrobacterium vectors, nematode specific genes were introduced into host plant.
D. Two RNAs being complementary to each other formed a double stranded RNA (ds RNA) that initiated RNAi and silenced specific mRNA of nematode.
Read the following statements and find out the incorrect statement
(i) Now-a-days the most commonly used matrix is agarose which is a synthetic polymer.
(ii) Larger the fragment size, the farther it moves.
(iii) We can not see pure DNA in UV light without staining.
(iv) DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel.
Given below are two statements :
Statement-I : Downstream processing includes separation and purification of the product after completion of the biosynthetic stage.
Statement-II : The downstream processing and quality control testing is same for all kind of product. In the light of the above statements, choose the most appropriate answer from the options given below :
Read the following statements:
A. Since DNA is hydrophilic molecule, it cannot pass through cell membrane.
B. Bacteria are made competent to uptake DNA by treating with monovalent cations like calcium.
C. To enable the bacteria to uptake recombinant DNA, the cells with recombinant DNA are incubated on ice followed by placing at 42°C and back on ice.
D. In gene gun, plant cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA.
Select the correct statements.
A,C, D
The given segment of DNA has restriction sites I and II, which create restriction fragments a, b and c. Which of the following band pattern produced by electrophoresis would represent the separation & identity of these fragments ? 
